Mean and variance of ratio estimators used in fluorescence ratio imaging

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چکیده

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Mean and variance of ratio estimators used in fluorescence ratio imaging.

BACKGROUND The ratio of two measured fluorescence signals (called x and y) is used in different applications in fluorescence microscopy. Multiple instances of both signals can be combined in different ways to construct different ratio estimators. METHODS The mean and variance of three estimators for the ratio between two random variables, x and y, are discussed. Given n samples of x and y, we...

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Fluorescence Ratio Imaging

In this paper, the properties of the mean and variance of three estimators of the ratio between two random variables x, y are discussed. Given n samples of x and y we can construct two different estimators. One is biased and the other is asymptotically unbiased. Using the noise characteristics (variance, covariance) a third, unbiased estimator can be constructed. 1. Introduction In fluorescence...

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ژورنال

عنوان ژورنال: Cytometry

سال: 2000

ISSN: 0196-4763,1097-0320

DOI: 10.1002/(sici)1097-0320(20000401)39:4<300::aid-cyto8>3.0.co;2-o